Aquaculture America 2020

February 9 - 12, 2020

Honolulu, Hawaii

ASSESSING GUT TRANSPORT OF METHIONINE AND LYSINE IN FAST-GROWING RAINBOW TROUT

 
 Melissa Marsing , Ken Overturf and Madison Powell
 
Aquaculture Research Institute, University of Idaho, Hagerman, ID 83332 USA
 

Future growth of aquaculture depends on the discovery of alternative sources of protein to feed fish.  Plant-derived proteins lack sufficient levels of two essential amino acids, methionine and lysine, for fish  dietary requirements. W e examined differences between a genetically selected line of rainbow trout that  grows more  rapidly on  a plant protein diet when compared to a non-selected line. We hypothesize  genetically  selected rainbow trout uptake these two limiting amino acids more efficiently in their gut via more numerous amino acid transporters as compared to non-selected fish. Amino acid uptake and  total amino acids were assessed  over time in plasma  using [13C] and [15N ] stable isotopes of methionine and lysine measured by LCMS .  Amino acid transporter expression in the gut was assessed using qPCR. R esults show amino acid uptake in rainbow trout selected for growth on a plant-protein diet is greater than the uptake observed in non-selected fish. Quantitative PCR of amino acid transporters in the proximal intestine  also  show increased expression stimulated  by lysine  over time consistent with levels amino acid of uptake.