Aquaculture America 2020

February 9 - 12, 2020

Honolulu, Hawaii

GLUTAREDOXIN 2 FROM Hippocampus abdominalis (HAGRX2) AND ITS POTENTIAL INVOLVEMENT IN CELL SURVIVAL AND IMMUNE RESPONSES

W.K.M Omeka*, D.S Liyanage and Jehee Lee
Department of Marine Life Sciences & Fish Vaccine Research Center,
Jeju National University, Jeju Self-Governing Province 63243, Republic of Korea
malithiomeka@gmail.com
 

Glutaredoxins are belongs to thioredoxin super family and possess thioredoxin catalytic motif. I n vertebrates, G rx1 and G rx2 consider as dithiol proteins whereas G rx3 and Grx5 are monothiols. According to previous studies, Grx2 perform critical functions in both development and redox homeostasis in fish. Therefore, HaGrx2 was characterized by bioinformatical, transcriptional and functional assays .  

Insilico analysis was performed by CLC main work bench version 8.0. Tissue distribution and immune challenge experiment (LPS, Poly I:C, E.tarda and S.iniea) was conducted  and performed q-PCR analysis. Further, FHM cell survival upon H2O2 stress was measured by MTT assay.

According to the insilico analysis, HaGrx2 possess typical glutaredoxin structure with CPYC thioredoxin active motif and showed highest identity (95.4%) with Grx2 from Hippocampus comes. HaGrx2 exhibit highest expression in skin and brain of healthy fish. Liver HaGrx2 showed significant upregulation toward poly I:C, E.tarda  and S.iniea . Further, HaGrx2 exhibit significant antiapoptotic activity against H2O2.

Grx2 is an enzyme that regulate many cellular pathways. As it is a redox enzyme, directly regulates the redox signaling during oxidative stress conditions. Also, it is known as a redox switch  for releasing mitochondrial ROS during innate immune responses.  Further, it can  regulate the mitochondrial energy producing complexes by deglutathionylation thus boost the metabolism and host defense mechanisms  indirectly  at normal and stress conditions. Therefore, Grx2 might play a vital role in cell survival, growth, proliferation and innate immune responses. Altogether, results in this study suggests that HaGrx2 actively involved in host  innate immune responses as well as cell survival during oxidative stress conditions.