Aquaculture America 2020

February 9 - 12, 2020

Honolulu, Hawaii

HEAT SHOCK PROTEIN 70 GENE EXPRESSION AND STRESS RESPONSE OF PACIFIC ABALONE Haliotis discus hannai TO TEMPERATURE, SALINITY AND AIR-EXPOSURE STRESS

 
Hyo Bin Leea , Soo Bin Jeonga, Sung Won Kangb, Dae Cheol Seob , Sol Min Wooa, Han Kyu Lim a,*
 a Department of Marine and Fisheries Resources
Mokpo National University
Jeonnam, 58554
 Republic of Korea
Limhk@mokpo.ac.kr
b Fisheries Seed Research Institute
Jeollanam-do Institute of O cean and F isheries Technology
Jeonnam, 59146
 Republic of Korea
 

Hsps play an important role in the cell' s response to a wide range of damaging conditions ,  and  they  are important f or  the  recovery and survival of organisms. An important member of  the Hsp family, Hsp70 is involved in chronic temperature acclimation and acute responses in aquatic animals. This study observed the expression regulation of the Hsp70 gene, and the changes in hemolymph glucose levels as a stress response in Pacific abalone (Haliotis discus hannai ) during exposure to water temperature shock, low-salinity and air-exposure. In the initial period of high temperature stimulation, the expression of Hsp70 in Pacific abalone muscle increased up to 4 times and then decreased at high temperatures for 6 hours. In the recovery phase, it increased rapidly to 8 times its normal levels . In contrast, there was no significant difference in Hsp70 expression under hypothermic stimulation. Under low salinity stimulation, Hsp70 increased rapidly up to 8 times and then decreased as much as  the  control in the recovery phase. The difference  from  air exposure stress was small compared to the control. The hemolymph glucose was measured and gradually increased under all stress conditions and then decreased during the recovery phase. Pacific abalone is  a cold water species, so it is not stressed at low water temperatures, but high water temperatures and low salinity  should be considered to be a varia bles in the rate of Hsp70 expression.