As known that food additives have been using for extending the shelf life of foods, for aroma and colour enhancer, to improve the structure and cooking properties of food, and to maintain and improve nutritional value. More than 10.000 chemicals are allowed to be added to food and food contact materials including fish food. Although an estimated 1.000 chemicals are used under a GRAS designation process, the others are only available on the basis of the Acceptable Daily Intake (ADI) values. The authorities/commissions authorized to determine the ADI value have continuously been opening the calls for toxicological data to re-evaluate the values and have published many re-evaluation reports. Some of these reports contain advice regarding the ADI values which should be re-evaluate and re-determinate continuously with new technological and current methods. The reports containing such recommendations raise the question of whether these values, which could affect the health of millions of people, are actually determined at the right limits. Here, telling about a new zebrafish modelling for evaluating the toxicity of food additives. Zebrafish embryos use only eggs with reserves of fat droplets in the yolk as a food source during the first five days of embryonic development. These animals, which are forced to live dependent on yolk, can be defined as "closed systems" in which food intake is not influenced by outside. During that time, adding any additive to yolk sac (to food) via microinjection, make these animals be a good model for detailed evaluating side effects or ADI limits of additives.
Keywords: Acceptable Daily Intake (ADI); microinjection; animal modelling