Crappie, from the genus Pomoxis, are North American, warm water Centrarchids. Both species in this genus, the black crappie (Pomoxis nigromaculatus) and the white crappie (Pomoxis annularis ), are popular and are of significant importance to the sportfishing and recreational fishing industries. Recently, the use of the hybrid, a first-generation cross between the black crappie male and white crappie female, has gained popularity. The hybrid has shown superior growth traits and has been known regularly outgrow the parental species. The hybrid also has greatly reduced reproductive capacity. This is considered an advantage by pond owners, as uncontrolled reproduction and overpopulation is a problem encountered with both parental species. Black and white crappie do not readily hybridize in the wild, and due to the villiform nature of the testes, males have to be sacrificed to obtain milt for in-vitro fertilization and hybrid production. The production of xenogeneic white and black crappie broodstocks would enable the production of hybrids by natural pond spawning. In xenogenesis, primordial gem cells (PGCs) from a donor are extracted and implanted on the gonadal ridge of a sterile host. The implanted PGC will colonize and produce germ cells from the donor in the gonad of the host. The aim of this study is to develop and characteriz e a new cell culture protocol for developing a xenogeneic triploid crappie. Primary cell culture protocol development in triploid crappie is underway and the results will be presented.