Studies in transcriptomes and genomes of cultivated species are increasing rapidly, looking for identifying and monitoring genes for improving productivity in aquaculture. Growth is an important trait widely studied in different species under captivity. Genes related to different GO categories associated with growth have been identified playing important roles in the growth of different fish species, but due to the complexity of this trait, influenced by genetic and environmenta l components, interactions between genes are not well known, moreover changes in gene expression profiles between species or developmental stages could also occur. In Peru, transcriptomes of larvae and juveniles of the cultivated flounder Paralichthys adspersus (Steindachner, 1867) have been recently obtained, and different genes related to growth have been identified in silico. In this sense, ten genes with differential expression levels observed by comparing liver and muscle transcriptomes from fast and slow-growth juveniles of P. adspersus were selected as candidates for monitoring growth during development and early juveniles stages, and the corresponding primers were designed. Sequences from supertranscripts data were used to design RT-qPCR primers , using the tool Primer-Blast from NCBI. Expected products ranged from 80 to 150 bp. Parameters like primer length from 20 to 22 bp, melting temperature from 50 to 60°C considering 1 °C of difference between them, and 40% to 60% of GC content in 3' extreme sequence, were also considered. Candidate primer pairs were analyzed using OligoAnalyzer from IDT Technologies, in order to avoid hairpins, homodimers or heterodimers. For all oligos designed, delta G values were higher than - 8 kcal/mol, showing instability of secondary structures in and between them. Finally, oligos selected were blasted against the Japanese flounder Paralichthys olivaceus genome and aligned with P. adspersus supertranscripts, demonstrating their specificity to a single gene in both species. Selected genes were identified mainly related to organ development and binding protein functions, in fish. These selected candidates will be tested on tissue samples from different larval stages and juveniles for further evaluation of their relative expression and identification of candidates for growth selection.