The present study aim is to compare the survival and growth of E. coioides larvae fed with calanoid copepod Acartia southwelli nauplii and enriched rotifers Brachionus plicatilis solely and in combination (numbers per mL) respectively. The experiment was conducted in early larvae of 2nd to 9th day of post hatch (dph). Monospecies of adult calanoid copepod A.southwelli (TI - 0.2 numbers/ml), A.southwelli nauplii 2 nos (TII) & 4 nos per ml (TIII) and Rotifer, Brachionus plicatilis 6 numbers/ ml (TIV) & 10 nos per ml (TV) and in combination of , A.southwelli nauplii and B. plicatilis (2 & 6 nos mL (TVI) and 4 &10 nos/ml (TVII)) in triplicates. Fatty acid composition of A. southwelli nauplii, microalgae enriched rotifer B. plicatilis and as reference livefeed, newly hatched Artemia nauplii were analyzed. The result shows, mean value of EPA in A.southwelli was (2.1 ± 0.1) which favours a high DHA: EPA ratio and there was a significant difference (P<0.05) between the estimated value of EPA between A.southwelli and the other two live feeds. DHA in A.southwelli shows mean value of higher (20.3 ± 0.7) than that of Artemia nauplii (0.2 ± 0.1) and B. plicatilis (3.1 ± 0.3). The total amount of SAFA was (40.1 ± 2.7) in A.southwelli and Artemia nauplii & B.plicatilis were (44.5± 4.2) (46.6± 3.6). Similarly, the number of monounsaturated fatty acids detected in A.southwelli was (19.2± 1.2) was much greater than B.plicatilis (17.2± 1.1). The total amount of PUFA in B.plicatilis (29.1± 2.3) was higher than A.southwelli (10.2± 0.8). The total amount of HUFA was found to be significantly greater (P<0.05) in the copepod A.southwelli (21.4± 0.2) than Artemia nauplii (7.2± 1.3) and B.plicatilis (7.1± 2.4). The mean survival rate of TVII and TVI (11.83±0.21 & 9.17± 0.15) was higher than all other treatments and shows significant (P<0.05) in early larval survival percentage. Further, the result indicates that early grouper larvae on 9dph has better growth TL (4.48±0.20 mm) in TVII and there was no significance difference (P>0.05) in all other treaments except in T-I. In conclusion, A.southwelli nauplii (N-I) as initial livefeed is rich in EPA/DHA ratio which has much influence on survival rate and shows better growth of E. coioides larvae. The calanoid copepod A.southwelli nauplii (N1) in combination with appropriate size rotifers (<100µ) could improve the orange spotted grouper larval production.