The current study was carried out for 147 days to assess the Vibrio count of Litopenaeus vannamei culture ponds from Vakapadu, Visakhapatnam District, Andhra Pradesh, India. Three ponds were selected, one is control and other two were experimental (Pond A and B). The physico-chemical parameters of the culture ponds were recorded by adopting standard methods. During summer crop the control ponds were harvested at 24.0 gm on 107th day and at 31.0 gm on 121st day for the year 2017 and 2018 respectively due to the incidence of Vibriosis disease. Where as in winter crop during 2017 the control pond was harvested at 5.5 gm on 47th day because of Vibriosis but in 2018 the control pond was harvested normally at 32.5 gm on 145th day, as this pond is free of Vibriosis. Experimental ponds were harvested normally in summer season at 30.5 gm on 124th day & 31.0 gm on 127th day during 2017 and 34.0 gm on 127th day & 35.5 gm on 127th day in the year 2018 respectively. In the winter season of year 2017, experimental ponds were harvested normally at 28.0 gm on 124th day as well as on 127th day. In the year 2018 winter, the experimental ponds were harvested at 36.0 gm at 146th day and 35.5 gm at 147th day respectively. This study suggests that the probiotics are the key agents which have a great impact on the reduction of total Vibrio count in culture ponds of L. vannamei.