A 42-day indoor feeding trial was conducted to evaluate the growth performance and feed utilization of juvenile white shrimp Litopenaeus vannamei fed with diets containing different supplement levels of papin enzymes. The commercial white shrimp feeds containing 36% crude protein were incorporated with graded levels of papin enzymes (0, 250, 500, 750 and 1000 mg kg-1) and used in the feeding trial. Shrimp (mean initial weight, 0.13±0.004 g) were fed three times daily ad libitum at an initial feeding allowance of 10% total body weight day-1. A completely randomized design was used in the study and shrimp were stocked at a density of 10 animals 20 L-1 black round plastic tank in triplicates. Results showed that the different levels of dietary papain enzymes significatly affected (p<0.05) on final body weight (5.3-5.5 g shrimp-1), survival (80-96.7%), and total moulting shrimp (36-48 times). In terms of weight gain, the diet with no papain enzymes and the highest papain enzyme inclusion level of 500 mg kg-1 diet seemed to have better performance (p<0.05) than 250 mg/kg diet. However, the shrimp fed diets containing papain enzymes at 500, 750 and 1000 mg kg-1 diets showed a better feed utilization (Feed Conversion Ratio (FCR) 4.5-5.3 and weight gains than those of shrimp fed the feeds containing papain enzymes at levels of 0 mg and 250 mg kg-1 diet (FCR 6.32-8.62; weight gain 4.31-4.92 g). The results indicated that dietary papain enzymes supplementation had a significant influence (p < 0.05) on feed utilization. Shrimp fed the 500-1000 mg algae extract kg-1 diet had a significantly better feed intake, FCR and weight gain (p < 0.05) than the shrimp fed the 0 mg and 250 mg algae extract. These results suggest that supplementation of papain enzymes at a dose of 500-1000 mg kg-1 can be used to get a better feed utilization performance (reduce 41.7% FCR and enhance 21.4% weight gain) white shrimp L. vannamei. However, a dose of 500 mg papain enzymes kg-1 diet is recommeded to add in diet to get a better FCR and growth performance of juvenile L. vannamei.