A study was conducted to investigate the effect of stocking density on growth performance and survival of Indian major carp Labeo rohita and Catla catla by installing net screen barrier system in Bishalakhi canal of Sundarban. The treatment had four stocking density 0.5 (T1), 1 (T2), 2 (T3) and 4 (T4) nos. per m2. Advance fingerlings with average weight of L. rohita 16.2±2.1 g and C. catla 14.8±1.6 g were stocked into 4 net screen barrier system at ratio of 1:1. The fishes were fed with a diet containing 32% crude protein and 5 % crude lipid at 4% of their body weight twice a day for 120 days. The final average weight of fishes were ranges from 80.09±6.8 - 91.27±8.7 g & 59.36±5.5 - 68.33±6.2 g and average daily weight gain ranges from 0.53±0.02 - 0.63±0.04 & 0.37±0.01 - 0.45±0.02 g day-1 for L. rohita & C. catla respectively. Fishes stocked with lower stocking density showed better growth performance than higher stocking density. The highest final average weight gain and average daily weight gain was noted at a stocking density of 0.5 nos. per m2 (T1). However, there was no significant difference between the treatment T1 and T2 (P< 0.05). Survival rate of fishes was not affected by stocking density (p<0.05). Based on growth parameters, it can be concluded that the most effective stocking density was at 0.5 nos. per m2. The study suggest for sustainable use of these unexploited resources.