The climbing perch, Anabas testudineus is an air-breathing fish having great consumer preference as a food fish and is considered a prime candidate species for aquaculture. Spawning success is an important issue while using hormones for captive induced breeding. Hlawga fish farm, Mingalardon Township, Yangon Region was used to carry out studies on the induced breeding of Anabas testudineus under varying brood stock ratios between May 2018 and November 2018. Six gravid females weighing 0.5 kg each and six reproductively mature males weighing 0.5 kg each were used for the three induced breeding trials in ratios of 2:1, 2:2 and 2:3 female/male respectively. Females were injected at a dosage of 0.50 mL ovaprium/kg body weight and 0.25 mL/kg body weight for males. Hatching starts after 24 hours of incubation and lasted for 6 hours. Dissolved oxygen, pH, ammonium ion, nitrate ion levels and temperature were monitored. Mean weight of eggs produced was 185.0g ± 65.0 with a relative percentage weight of eggs to body weight of 36 to 43%. The lowest pseudo-gonadosomatic index of 41.5 ± 6.5 was recorded in treatment 1, while the highest value of 51.0 ± 3.0 were recorded in treatment 2. The effect of the varying brood stock (female/male) ratios in all trials were not significantly different at (p < 0.05) as indicated by the number of fertilized eggs, number of hatched eggs, % larval production, and survival.