White Spot Syndrome Virus (WSSV) is a major shrimp disease in South East Asia. It causes high mortality rates and enormous economic losses to the major shrimp farming countries. In the present study, shrimp samples from three trap and hold shrimp farming systems in Pyapon Township, Ayeyarwady Region, Myanmar, were selected as study sites and occurrence of WSSV in the farms were scanned from December, 2018 to March, 2019. Ten samples of Penaeus monodon were collected monthly from each study site before they were harvested to the market. Pleopods from each individual were collected and preserved separately in 1.5ml micro-centrifuge tubes with 70% ethanol. They were transported to the Laboratory of Aquatic Bioscience, University of Yangon to scan forWSSV virus using nested PCR. No positive samples were detected in December 2018 and January 2019. However, in February, 6 out of 10 samples from Farm 1 and 4 out of 10 samples from Farm 3 were positive in 2nd step PCR. Typically, the positive in 2nd step PCR means infected shrimps were in the latent or carrier stage. The result in the present study indicated that WSSV in Pyapon shrimp farms have only carrier stage.