The objective of this study was to evaluate the growth, survival, immune potentials of Indisn white shrimp Penaeus indicus reared in biofloc systm with addition of customised beneficial bacteria. The experiment was conducted in tank (1 ton) based systems in triplicates. A total of nine probiotics were isolated and screened from successful BFT runs following standard protocol. Respective culture of these bacterial strains and their consortium with carbon sources were fermented for 24 hrs in sterilised sea water and applied. Optimum C:N ratio (12:1) was maintained for the heterotrophic bacterial production by adding required quantity of mixed C sources (wheat flour, rice flour, ragi flour, maida, corn flour, molasses) per amount of nitrogen generated after assimilation of feed given per day. The bacterial consortium group, followed by certain species like Virgibacillus showed higher growth (p≥0.05) and enhanced immunity than other groups. The total suspended solids (TSS) level was maintained at around 200 mg/litre in BFT tanks. Shrimp survival was approximately varied from 80--95%. Net shrimp production performance was 35% higher in the BFT tanks (ABW=10.89±1.2) than in the control tanks (7.56±1.78) confirming the utilization of biofloc by shrimp (Penaeus indicus) as food. The immunological parameters like total haemocyte count, total protein in serum, pro-phenol oxidase (proPO) activity, lysozyme activity were found to be significantly higher in BFT groups than that of the control groups. The gene expression study showed relative expression levels of potential immune genes were significantly up-regulated in shrimps grown in these customized biofloc groups. This study imply that the customization of biofloc based system with suitable bacterial consortium elucidates the production performances as well as immune status of the rearing animals.