Nine semi-purified diets containing three levels of protein (35%, 40% and 45%) and three levels of lipid (6%, 8%, 10%) were fed ad libitum to climbing perch Anabas testudineus fingerling (1.64±0.01 g) in triplicate groups (10 fish/replicate) for 90 days to determine optimum dietary protein and lipid levels for this species. Fish were stocked in 27 flow-through fiber-reinforced plastic tanks with 50 L of water (10 fish/ replicate). In every 15 days interval, the fish were batch-weighed to determine growth. The dietary protein and lipid levels individually or in combinations had significant effects (p<0.05) on weight gain, specific growth rate (SGR), feed conversion ratio (FCR), protein efficiency rate (PER) and protein productive value (PPV) of fish. The fish fed 40% protein had significantly higher (p<0.05) weight gain, SGR, PER and PPV and lower FCR (p<0.05) as compared to higher (45%) and lower (35%) protein fed groups. Similarly, the fish fed different lipid levels had significantly higher (p<0.05) weight gain, SGR, PER and PPV and lower (p<0.05) FCR in medium lipid fed group (8%) than the lower (6%) and higher (10%) dietary lipid fed groups. The interactive effect of dietary protein and lipid indicated that the fish fed with 40% protein and 8% lipid had significantly higher (p<0.05) weight gain, SGR, PER and PPV and lower (p<0.05) FCR than the other dietary protein and lipid fed groups. The dietary protein and lipid levels either individually or in combinations had significant effect (p<0.05) on whole body chemical composition (moisture, crude protein, ether extract, ash and energy) in climbing perch fingerling. Based on higher growth and nutrient utilization, the optimum dietary protein and lipid levels of climbing perch fingerling are 40% and 8% diet, respectively.