An experiment was conducted to study the effect of dietary ethanolic root extract of Asparagous racemosus (Satawari) on growth performance, nutrients utilisation and biochemical composition of carcass in Labeo rajasthanicus fingerlings. Four iso-nitrogenous and iso-caloric (30 % crude protein and 390 kcal / 100 g) diets were prepared with the graded level of ethanolic root extract (RE) of Satawari 0%, 0.01 %, 0.015 %, and 0.02 % and designated as control, T1, T2, and T3, respectively. Fishes were fed twice daily for ninety-days following completely randomised design. At the end of the feeding trial, growth performance, nutrient utilisation, carcass composition and digestive enzyme activities were considered as response parameters. Significantly higher growth rates (weight gain %, specific growth rate), nutrient utilization (protein efficiency ratio, lipid efficiency ratio) and feed conversion were observed in T1 group compare to other dietary groups. The average carcass composition was found to vary (P<0.05) among the different dietary groups. The digestive enzymes viz. protease, lipase and amylase activities were significantly higher in fishes fed with 0.01% RE than other dietary treatments. The present study revealed that 0.01 % ethanolic root extract of Satawari improves growth, nutrients utilisation indices and feed conversion without any adverse effect.