Asian-Pacific Aquaculture 2019

June 19 - 21, 2019

Chennai Tamil Nadu - India

WHOLE GENOME SEQUENCING AND DE NOVO DRAFT ASSEMBLY OF INDIAN WHITE SHRIMP Penaeus indicus

M.S. Shekhar*,  K. Vinaya Kumar, J. Ashok Kumar, C.P.Balasubramanian, K. Karthic, K.K. Vijayan
 
Genetics and Biotechnology Unit, Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, R.A Puram, Chennai
 
 *msshekhar@hotmail.com
 

The shrimp genome is large, with high repetitive content and a very complex genome for sequencing.  A high quality draft whole genome assembly of Indian white shrimp Penaeus indicus has been obtained in the present study using PacBio-Sequel platform. The shrimp genome size which was estimated to be 2.47 ± 0.04 pg by flow cytometry analysis, could be successfully assembled using canu assembler. Proportion of 95.54 % of shrimp genome could be assembled which generated 83892 no. of contigs, 2368024291  bp total assembled length, and  54266 bp N50, with largest contig of 1278629 bp  with 34.84%  GC content. BUSCO analysis showed high level of genome assembly completeness with some fragmentation. This high quality assembled genome of P. indicus reveals basic genomic composition and will be useful as a reference genome for comparative genomics of other penaeid shrimp species and genetic improvement programmes of P. indicus.