Gynogenesis is a method followed in aquaculture for producing all female population without genetic contribution of male gametes. Gynogenesis is used in aquaculture to generate monosex populations with higher economic value. In the present study gynogenesis was induced in Asian catfish, C. batrachus using UV irradiated heterologous sperm of M. armatus. The survival of embryo and hatching percentage decreased with the increase in UV irradiation dose. The haploid syndrome characterized by short tail and enlarged cardiac cavity was also observed. Chromosome preparation from these UV irradiated batches showed that these hatchlings were haploid with n=25 chromosome. The cold shock temperature of 1ºC yielded overall maximum of 87% gynogenetic individuals at the embryological age of 4 minutes when the activated eggs were shocked for 3 minutes duration. The diploid gynogen had a morphological appearance as that of Clarias batrachus. This clearly indicates that all the hatched out larvae were gynogens, because eggs of C. batrachus and sperm of M. armatus did not yield viable hybrid hatchlings. The conformation of diploid gynogens were based on the chromosome count of 2n=50.