SURVEILLANCE OF EHP IN MAHARASTRA AND GUJARAT

The occurrence of EHP in Maharashtra and Gujarat Penaeus vannamei was assessed. In the present study, shrimp samples from two districts of Maharashtra (Thane and Raigad) and two districts of Gujarat (Navsari and Surat) were collected over a period of two years (February 2016 to April 2018). A total of 4513 shrimp samples were assessed for the presence of EHP by Molecular characterization. Out of shrimp samples collected, about 45% samples were positive for EHP. The screening of EHP was done by single step and nested PCR targeting spore wall protein gene (SWP) of EHP resulting in product size of 514 bp and 148 bp for EHP respectively.