The study was conducted to evaluate the flocculating potential of spentwash as a carbon source enriched with the consortium of four Bacillus species such as Bacillus subtilis, Bacillus megaterium, Bacillus infantis and Bacillus cereus isolated from biofloc culture water of GIFT Tilapia. Bacterial cultures after 24hrs of incubation were centrifuged at 5000rpm and the resulting cell pellet was resuspended in saline buffer. Bacterial suspension was adjusted to 1 OD and the bacterial consortiums were mixed with spentwash. The study was conducted in 150 litres FRP tanks for a period of 45 days. The experimental group includes spent wash enriched with bacterial consortium (T1), Spentwash supplemented with yeast and bacterial consortium (T2), spentwash and rice bran (T3) and spentwash (T4) as control. The C/N was maintained at the ratio of 15:1 for floc development throughtout the experiment. pH, ammonia, dissolved oxygen, temperature; nitrate, nitrite, magnesium and calcium were found to be significantly different between the experimental groups. Floc parameters like floc volume, porosity, settling velocity, TS, TSS and TDS were monitored on weekly basis along with total bacterial count and were significantly different between control and treatments. The rapid increase in floc volume was found in T2. The total heterotrophic bacterial count was significantly higher in T2 followed by T1. The findings of the present study confirmed that the distillery spentwash enriched with yeast and bacterial consortium (T2) found to have higher flocculating ability for the maintenance of optimum water quality parameters with a denser heterotrophic bacterial load.