Effect of photoperiod on growth and survival of Clarias batrachus (Linnaeus, 1758) larvae was studied. A 50 days experiment was conducted in plastic tubs with 5L of water. Four days old C. batrachus larvae (weighing 13.2mg) after yolk sac absorption were randomly stocked in triplicates in fifteen plastic tubs at a stocking density of 40 larvaes/litre under five different photoperiodic conditions: 24L:0D (T1), 18L:6D (T2), 6L:18D (T3), 0L:24 (T4)), 12L:12D ©. Average body weight (542.60a±11.89 mg) and specific growth rate (SGR) (7.43a±0.04) were highest for larvae reared in 6L:18D photoperiodic condition and were significantly (p<0.05) different from other treatments. Percentage weight gain (PWG) obtained in 6L:18D photoperiodic condition was significantly(p<0.05) higher than other treatments. Average body weight, SGR, PWG were lowest for larvae reared in 24L:0D photoperiod. The allometric coefficient b obtained is higher for T3 (6L:18D) and T4 (0L:24D) which indicates that more growth potential than larvae in other treatments. Regression models were developed for body weight and total length at different days and best models were chosen based on the reduction in error sum of square and increase in R2. Polynomial 2-parameter model indicated the best fit to explain and predict body weight and body length of C. batrachus larvae with increase in age. Higher % of survival was found in 6hrL:18hrD and 0hrL:24hrD in comparison to all other treatments. Higher growth and survival recorded in 6L:18D can be attributed to better feed intake, higher melatonin synthesis and suppression of swimming activity and stress in dark condition. The hatchery managers can utilize the model for predicting the growth of larvae under different ages and also will be useful for calculating biomass at the various growing period of the larvae to optimize feeding. The present study concludes that light restriction (6L:18D) may be recommended as a simple low cost technique to maximize the hatchery production of C. batrachus larvae.