Introduction
Intervention of fisheries knowledge in human health through innovative idea of insulin production. Diabetes currently affects more than 371 million people worldwide and is expected to affect 552 million by 2030. There is every possibility for producing insulin through hybridising rDNA technology and fisheries science. By adopting this technique using Indian major carps as bio reactors, India can rank top in insulin production and can attain self-sufficiency in terms of export.
Methodology
The following optimum conditions are maintained, temperature - 37 ⁰C, growth media - LB broth, pH 5.5.5-8.5, generation time-20-40 min. The multiplication takes place by means of inoculating E. coli colonies into about 4 ml cultures, and are freshly inoculated into larger cultures of 500 ml. E-coli is administrated into fish feed, 10 ml was centrifuged at 500 g/10 min washed in PBS. Then added to 5 g feed pellets, mixed and air-dried at room temperature. Fishes were fed twice a day for 2 to 5 days. Colonization by adherence of E-coli to the intestine of fishes. This is due to fimbrial and non-fimbrial adhesions. Adhesins - Bfp, Intimin, flagella, LifA/Efal. Then the nutrient requirements of E-coli are essential amino acids, C, H, O, N, S. P, K, Mg, Fe, Ca, Mn, and traces of Zn, Co, Cu, and Mo are administrated. Environmental conditions are maintained at temperature - (32-33⁰ C), aeration is given, feeding frequency is twice a day, water exchange is 100%. The insulin is secreted by E-coli in the fish gut in the sequence of 153 nitrogen bases - 63 in A chain, 90 in B chain. Then it is extracted by mechanical filtration, chemotrophism and centrifugation at 3000g for about 5 min. The purification is done by means of HPLC and Immobilised metal Affinity Chromatography
Result
The fishes used are carp varieties like rohu. As, they are eurythermal and bentho-pelagic, suitable for adopting this technique.
Conclusion
In India, total annual expenditure by patients on diabetes care was, on average, INR 10,000 (US $227). Therefore, the estimated annual cost of diabetes care of India would be approximately 180,000 million rupees. Hence by adopting this scientific technique we can reduce economic burden of India.