EFFECT OF DIFFERENT DIETARY HERBAL MEALS ON IMMUNE-PHYSIOLOGICAL RESPONSES IN Labeo rohita (HAMILTON, 1822) FINGERLINGS

The present study was carried out in order to evaluate the effect of the locally available different herbal meal on digestive, metabolic and antioxidant or stress enzyme activities, biochemical status and immunological responses in Labeo rohita fingerlings. Eleven experimental diets namely C (control without herbal meal), FSM1 (Fennel seed meal), CRSM2 (Coriander seed meal), CMSM3 (Cumin seed meal), FGSM4 (Fenugreek seed meal), TRM5 (Turmeric root meal), BPFM6 (Black pepper fruit meal), ZRM7 (Zinger root meal), BLM8 (Bay leaf meal), DOBM9 (De-hulled onion bulb meal) and DGCM10 (De-hulled garlic clove meal) were used at 1% inclusion level. Four hundred and ninety-five (495) acclimated fishes (average body weight of 6.45 ± 0.016g) were randomly distributed in eleven experimental groups with triplicated under each at the stocking density of 15 fishes per tank for fifteen days.

After fifteen days feeding trial, the overall result of digestive and metabolic enzyme activities was found more influential diet containing FGSM4, TRM5, ZRM7, DOBM9 and DGCM10 in the issue of intestine, liver and muscle tissue. Antioxidant enzyme superoxide dismutase and catalase activities were having wide-ranging impact in liver and gill tissue in the diet of CRSM2, CMSM3, TRM5, BPFM6, ZRM7, BLM8, and DGCM10. Blood parameters (Total leucocyte count, serum protein, albumin, globulin, and NBT) were assessed and found to be promising for improving the innate immune system in the diet of TRM5, ZRM7, and DGCM10. Considering digestive and metabolic enzymes, antioxidant enzymes activity and enhance the innate immunity function, it revealed that inclusion of Turmeric, Zinger, and Garlic in the practical feed of Labeo rohita fingerling  may improve growth, antioxidant status and modulate innate immune system for healthy fish.