The present study investigated the sedative effect of ethanolic extract of Datura stramonium leaf on freshwater fish in Cyprinus carpio. The study consisted of seven groups, including control with doses ranging from 0 (control group) and 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 ppm in treatment groups. The results manifested the statistical difference between various groups subjected to D. stramonium leaf extract (p<0.05). The treated fish were anaesthetized within 15.43 minutes and recovered in the short time of 14.30 minutes (p<0.05). The induction time tends to decrease in response to increase in the concentration of ethanolic extract of D. stramonium leaf (p<0.05). The findings of the present study displays that D. stramonium plant extract could be used as a natural sedative to fish as they are cheaper and have little hazardous effect on fish. The results suggest that ethanolic extract of D. stramonium @ 2-3ppm could be used as a sedative with negligible mortality in common carp (C. carpio).