Pollution is the unfavourable alteration of our environment, largely because of human activities. Environmental pollution, especially water pollution, has been increasing at an alarming rate due to rapid industrialization, civilization and green revolution. In the present study the LC50 value of chlorpyrifos in the shrimp, Litopenaeus vannamei during 96 hrs was estimated to be 0.060 ppm. The experimental gill tissue exhibited epithelial lifting, edema, necrosis, fusion of secondary lamellae and hemorrhage. The deceased hepatopancreas revealed infiltration, formation of large lumen and disappearance of haemocytes. The pathologic symptoms like atrophy, necrosis, wavy appearance, accumulation of granular material in between muscle fibres, fragmentation, loss of muscle structure, appearance of basophilic deposits were displayed in the muscle tissue