A feeding trial including diets with three protein levels (25%, 30% and 35%) and three lipid levels (3%, 6% and 9%) was conducted to investigate the digestive enzyme activity ( Amylase, Lipase, Protease) in pearl spot fingerlings (average initial weight:12.76 ± 0.04 g). Fish were randomly distributed into 18 glass tanks (100 litre capacity). The fishes were fed thrice daily at 3% of their body weight. Every fortnightly all the fishes were weighted and redefine the feed ration. During the trial the digestive enzyme activity like amylase, lipase, Protease was analyzed. Amylase, protease found to be increased by increasing protein levels was (12.1±0.56 µg of maltose liberated mg-1 tissue protein h-1 at 37 0C), (22.1±0.62 µg of leucine liberated mg-1 tissue protein h -1 at 37 0C), but no significant (P>0.05) difference was observed. And lipase enzyme activity was found to be higher in fishes was (47.8 ±0.42 µg of α-napthol reduced mg-1 tissue protein h-1 at 37 0C) fed with 9% lipid was significantly (P < 0.01) higher than that of the other groups. Feed with higher inclusion level of protein (35%) has higher protease activity than other treatments and higher lipase activity was noticed with feed with 9% lipid level, resulted in increased stress response in experimental fishes. The results of this study indicated that Amylase, Protease enzyme activity was found to be higher with higher inclusion level of protein in feed. And higher Lipase activity was found in higher lipid inclusion feed.