Brachionus plicatilis were mass cultured in brackish water using 2.4 ton capacity FRP tanks. Utilization efficiencies for different food combinations using baker's yeast, chlorella sp and mixed algae for rotifer production were evaluated. Once the chlorella sp and mixed algae reached 4.5-5 x 106cells/ml the rotifers were inoculated into the treatment as (T1), and the baker's yeast was added to the treatment (T2) at the rate of 1 gram per liter followed by mixed algae as treatment (T3). An experiment was carried out in triplicate. Under optimum conditions, rotifer density was reached 290.00a±0.77 cells/ml at chlorella sp fed tank on eight days, Baker's yeast fed tanks 216.67b±0.08 cells/ml followed by mixed algae fed treatment was found to be around 180c±0.26 cells/ml. After eight days of culture, it was noticed that the cell density got a reduction in all treatment tank. Hence the present study could draw a conclusion that the chlorella sp can be used as feed for rotifer (Brachionus plicatilis) to attain maximum cell density followed by baker's yeast. And showed a highly significant difference among the treatments (p<0.05).