Road transport of live fish is a complicated interaction between fish, environment, technical equipment and various human factors, which needs an effective quality assurance system. Verification of correct biomass and capacity, appropriate supply and control of water and oxygen, panic and trip response of shipped stock are the major influential factors affecting the welfare of live fish during road transportation. Large numbers of transportation units are available for industry scale use but very less amount of technical support is available for conventional transport technique for small scale use. On-trip functioning life-support device can be a very effective and economic option to provide a quality maintenance system to get higher market value of live fishes. Life-support systems are typically composed of special purpose built insulated tanks and fitted with provisions for monitoring and control of critical water quality parameters like temperature, dissolved oxygen (DO) levels, pH, carbon dioxide (CO2) and ammonia (NH3) concentrations. Closed-circuit or recirculating systems are very efficient because of adaptability to function properly in all sub-optimal situations and also in case of shock loading. Laps in technical design and lack of prior knowledge for monitoring of each component mainly the filtration and aeration unit can only be the limiting factor in the performance of such systems. Proper installation of such innovative life-support systems could open the doors to various possibilities like expansion of existing transport opportunities, accommodation of higher stock density, improved market value and improved ability to withstand market fluctuation and also expansion into more distant markets.