Moina micrura, a cladoceran species, is considered to be one of the best live food organisms for rearing the young larval stages of fish and prawn. In view of importance of the species in hatchery practices, study was undertaken to develop continuous mass production technology for M. micrura in RAS based advanced culture system using Chlorella vulgaris as media at Aquatic Rainbow Technology Park (ARTP), TNJFU, Madhavaram. To feed M. micrura, Chorella media was developed in 6 ton tanks by fertilizing with the GNOC, urea and SSP @ 25%, 1% & 0.5% per ton respectively. To maintain continuous culture of Chlorella culture tanks were re-fertilized fortnightly. Mass production of M. micrura was produced in 2, 4 and 6 tone capacity advanced culture tanks facilitated with bottom line aeration by feeding with Chlorella (50000/ml) 3x/day as media. Initially culture tanks of M. micrura were inoculated at density of 6-10 ind/ml and the highest density of 42±2 ind/ml was observed on 8th day of the culture period. To maintain steady maximum density and continuous production partial (30%) harvesting practice was followed daily. The present study achieved success in mass production of M. micrura on commercial scale with reuse of water which can be helpful to replace Artemia (high input cost) in fish and shrimp hatcheries.