Puntius chonchonius popularly known by English name "Rosy barb" is native to Ponds of North-eastern Bengal but widely distributed all over the India. Rosy barbs are most preferred fish due its bright colour, active movement ad peaceful behaviour. Demand for this fish very high and hatchery seeds have many advantages over the wild ones thus it becomes necessary to standardize the doses of synthetic hormone for artificial propagation of the fish and develop an induced breeding protocol. In the present study captive breeding trials were conducted administrating HCG and WOVA-FH at different (0.1, 0.2, 0.3, 0.4 and 0.5 ml/Kg body weight) doses orally through feed. Healthy brooders of Rosy barbs were collected from local ornamental fish market (Kolathur, Chennai), In captive brood stock were successfully raised in RAS facilitated FRP tanks with a nutritionally balanced fish diet (38 % protein) and intermittently fed with Tubifex worms for a period of two months. Both HCG and WOVA-FH fed fishes responded positively on 4th day of feeding. P. chonchonius is egg scatters, eggs (0.9-1.1 mm) are demersal observed to be attached on the bottom of the tank. Treatment fed with WOVA-FH at 0.3ml/kg body weight showed better performance in terms of fecundity (120±12/ female), Fertilization rate (78%±1.0) and hatching rate (84%±0.5). Standardization of nursery rearing of P. chonchonius was carried out in 1 ton capacity FRP tank by feeding with live feed and weaning to the formulated feed for 60 days. According to the mouth size fish was fed with rotifers, cladocerans and cyclopes from 3rd - 8th dph(day post hatching), 9th -17th dph and 18th - 29th day respectively. Present study infers that WOVA-FH is WOVA-FH is ideal synthetic hormone to increase the production and yield by hatchery bred seeds.