Surrounded by the sea on three sides, fish farms were active in Korea, especially in the South Sea and the West Sea. The farms were mainly adjacent to the land so that they can be easily affected by pollutants coming from the land. Several studies have been conducted on the distribution of the indicator bacteria (Escherichia coli, Fecal streptococci) in the aquaculture, there is a lack of information on susceptibility to antibiotics. Seawater samples were collected from off the coast of Seosan, Wando, Yeosu, Tongyeong, and Namhae(West Sea; Seosan, South Sea; Wando, Yeosu, Namhae, Tongyeong), where fish farms were densely populated, in April, June, August and October 2018. Antibiotic susceptibility testing was performed based on CLSI guideline M100-S25 and using 16 antibiotic agents. They include Ampicillin (AMP), piperacillin (PRL), ticarcillin (TIC), amoxycillin/clavulanic Acid (AMC), cephalothin (KF), cefuroxime (CXM), cefoperazone (CFP), ciprofloxacin (CIP), nalidixic acid (NA), gentamycin (CN), streptomycin (S), tobramycin (NN), tetracycline (TE), oxytetracycline (OT), chloramphenicol (C), trimethoprim/sulfamethoxazole (SXT). Escherichia coli (n=65) was most and least abundant seawater samples taken from Seosan (40%), Wando(35%) and Yeosu (2%), respectively. And Fecal streptococci (n=136) was most abundant in Seosan (47%) and Namhae (26%), but Tongyeong was not detected. Results of identification of 10 strains to Fecal streptococci, Enterococcus sp. was the most abundant, followed by E. faecalis, E. hirae, and E. Casseliflavus. Approximately 57% of Escherichia coli was resistant against AMP, followed by KF, TIC, and S, respectively, 52%, 41%, and 40%. The results show that the indicator bacteria from this study are not related to aquatic animals but to the pollutants emitted from the land, when using antibiotics in farms, the use of antibiotics that are highly resistant should be reduced.