DIVERSITY OF BACTERIAL COMMUNITY IN THE INTESTINE OF THE LARGE YELLOW CROAKER Larimichthys crocea

The large yellow croaker Larimichthys crocea is a commercially important marine fish and is mainly farmed in the southeast coast of China. We used Illumina high throughput sequencing platform to sequence the V4 region of 16S rRNA gene of the bacterial community in the intestine.

The gastric caecum, pyloric stomach, pyloric caecum, rectum, small intestine and other digestive tissues from 8 fish cultured in the same cage were collected and analyzed. There existed obvious differences of the bacterial communities between the small intestine and other tissues, especial the gastric cecum. Analyses of molecular variance and principal co-ordinates showed that the diversities of intestinal bacterial communities between the cultured group and wild group were significantly different. The classes of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Clostridia and the genus Bacteroides were the dominant bacterial communities. 12 species of bacteria were identified as the core intestinal bacterial community, the occurrence probability of which exceeded 60% in each group (Fig.1).