Pearl spot, Etrophus. suratensis average weight 20.12±2.29 g were collected from Pulikat Research Center of Tamil Nadu Dr. J. Jayalalithaa Fisheries University. Known volumes of 2-phenoxyethanol (500 µL/L,875 µL/L, 1000 µL/L and 1125 µL/L and 1250µL/L) were initially mixed with water (30 mL) in a reagent bottle (50 mL) and then stirred to disperse the chemical to form small droplets before adding to anaesthetic induction tanks. Three stages of induction (I-1, I-2, I-3) and three stages of recovery (R-1, R-2, R-3) were considered in E. Suratensis and the data were observed for each fish separately by using stopwatch. Mean induction time and recovery time of anaesthesia were compared among treatments using one-way ANOVA.
The longest time for induction was observed at 500 lL−1 (237.6±7.99 seconds), while the shortest time occurred at 1250 lL−1 (46.4±2.97 seconds) (Table-1). The longest time for recovery was observed at 500 lL−1 (180.4±3.51 seconds), while the shortest time occurred at 1250 lL−1 (44.8±2.86 seconds) (Table-2).
The results indicated that 2- phenoxyethanol can be used as an efficient anaesthetic for the handling of E. surantensis and the lowest effective concentration to induce anaesthesia is 500 lL−1.