WWW.WAS.ORG • WORLD AQUACULTURE • JUNE 2024 33 Further validation of the assay using a larger sample size and diverse geographical locations would enhance its applicability and robustness. Additionally, the integration of microfluidic systems or portable devices could streamline sample processing and analysis, enabling on-site detection in resource-limited settings. Moreover, exploring the potential for multiplexing to simultaneously detect multiple pathogens in a single assay would offer added value for comprehensive food safety monitoring. Overall, continued research and development efforts are warranted to optimize and refine our colorimetric detection platform for broader implementation and impact in public health and food safety initiatives. Conclusions The increase in global consumption of seafood highlights the critical necessity for swiftly detecting pathogens like V. parahaemolyticus. Hence, there is an imperative need for precise, rapid, and straightforward methods capable of efficiently screening pathogens. The developed method demonstrates detectability of the colorimetric signal at concentrations as low as 101 cfu mL-1 using a spectrophotometer and 103 cfu mL-1 through visual inspection. Moreover, extending the polyadenine length to 10 nucleotides notably reduces background signaling, achieving a relative intensity of 3.07 ± 0.23 arbitrary units (a.u.). Positive samples exhibit consistent relative intensity (OD410 = 0.55 ± 0.08) even after 120 minutes of incubation. This method presents a promising tool for the rapid and reliable detection of V. parahaemolyticus in aquaculture settings, enabling timely intervention measures to mitigate the risk of contamination and ensure the safety of aquatic products. Considerably, the HRPzyme-integrated PCR-based platform offers a sensitive, rapid, and cost-effective method for detecting Vibrio parahaemolyticus. This detection approach is particularly beneficial for resource-poor areas, providing a valuable tool for ensuring seafood safety and preventing foodborne illnesses. Future research will focus on the integration of this detection method into portable devices for field applications, enhancing its usability and accessibility. Notes Ali Parsaeimehr, Gulnihal Ozbay*, Department of Agriculture and Natural Resources, College of Agriculture, Science, and Technology, Delaware State University, Dover, DE 19901, USA * Corresponding author: gozbay@desu.edu References Ahmad, M. Sharma, P. Kamai, A. Agrawal, A. Faruq, M. and Kulshreshtha, A. 2021. HRPZyme assisted recognition of SARSCoV-2 infection by optical measurement (HARIOM). Biosensors and Bioelectronics, 187: 113280. https://doi.org/10.1016/j. bios.2021.113280. Alarcón Elvira, F. Pardío Sedas, V.T. Martínez Herrera, D. Quintana Castro, R., Oliart Ros, R.M. López Hernández, K., Flores Primo, A. and Ramírez Elvira, K. 2020. 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World Health Organization. 2022. WHO global strategy for food safety 2022-2030: towards stronger food safety systems and global cooperation. https://www.who.int/publications/i/ item/9789240057685. The increase in global consumption of seafood highlights the critical necessity for swiftly detecting pathogens like V. parahaemolyticus. Hence, there is an imperative need for precise, rapid, and straightforward methods capable of efficiently screening pathogens.
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