WWW.WAS.ORG • WORLD AQUACULTURE • JUNE 2023 55 sustained-release GnRHa implant. Aquaculture 219:841-858. Nelson, M.D., C.C. Koenig, F. Coleman and D.A. Mann. 2011. Sound production of the red grouper Epinephelus morio on the West Coast Florida Shelf. Aquatic Biology 12:97-108. Schärer, M.T. M.I. Nemeth, T.J. Rowell and R.S. Appeldoorn. 2014. Sounds associated with the reproductive behavior of the black grouper (Mycteropera bonaci). Marine Biology 161:141-147. Suquet, M., C. Dreanno, C. Fauvel, J. Cosson and R. Billard. 2000. Cryopreservation of sperm in marine fish. Aquaculture Research 31(3):231-243. Valenzuela-Gutiérrez, R., A. Lago-Lestón, F. Vargas-Albores, F, Cicala and M. Martínez-Porchas. 2021. Exploring the garlic (Allium sativum) properties for fish aquaculture. Fish Physiology and Biochemistry 47:1179-1198. TABLE 1. Information related to Mycteroperca bonaci biometrics, gender identification, and comments during fish delivery to the College of the Florida Keys – Southernmost Marine Aquaculture Research & Training (SMART) Center on Aril 8, 2022. Date ID Tag Tank Cannulated Length Weight Comments Color No. (Y/N) (cm) (kg) 5/17/21 BLK-1 No Tag NA N 76.2 5.4 BLK-1 was only kept in outdoor systems; BLK-1 perished on 2/17/22; ovaries were observed. 12/13/21 R3 (BLK-2) Red (R) 3 Y 61 2.5 BLK-2 was caught with the net from tank 3 and placed in the anesthesia bath for 25 minutes until fully sedated. Noticable external injuries include split or frayed tail rays and torn dorsal spines aquired during net transfer. Cannulation unsuccessful. 4/8/22 P1 Pink (P) 1 Y 66 4.7 No noticable external injuries. Cannulation unsuccessful. 4/8/22 O1 Orange (O) 1 N 62 3.2 No noticable external injuries. Cannulation not attempted. 4/8/22 Y1 Yellow (Y) 1 N 48 1.3 No noticable external injuries. Cannulation not attempted due to length (<60cm). 4/8/22 P2 Pink (P) 2 Y 61 3.4 Noticable external injures include fraying of the tail. Cannulation successful, but eggs are not viable. 4/8/22 O2* Orange (O) 2 N 52 2.0 Noticable external injuries include scarring around the dorsal fin and fraying of the tail. Neosporin (topical antibiotic) was added to scarring. Cannulation not attempted due to length (<60cm). *Recorded length dictates female should be small (Y) class range, but was tagged as medium (O). 4/8/22 Y2* Yellow (Y) 2 N 53.5 2.1 Noticable external injuires include scarring around the dorsal fin and along her underside and fraying of her tail. Cannulation not attempted due to length (<60cm). *Recorded length dictates female should be medium (O) class range, but was tagged as small (Y). 4/8/22 P3* Pink 3 N 55.8 3.3 Noticable external injuries include scarring along the left side of the body, damage and skin irritaion to the tail and dorsal fin, and pink irritation and fraying at the tip of the left pectoral fin. Cannulation not attempted due to length (<60cm). *Recorded length dictates female should be medium (O) class range, but was tagged as Large (P). 4/8/22 O3* Orange (O) 3 N 57 3.1 Noticable external injuries include scarring under the chin. Cannulation not attempted due to length (<60cm). *Recorded length dictates female should be Large (P) class range, but was tagged as medium (O). 4/8/22 Y3 Yellow (Y) 3 N 50.5 1.9 Noticable external injuries include missing dorsal spines, tail fraying, and curved operculum on both sides. Cannulation not attempted due to length (<60cm). ID = the first letter of the tag color and the tank number (e.g. Pink tag in tank 2 is P2)
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