36 MARCH 2022 • WORLD AQUACULTURE • WWW.WA S .ORG TABLE 2. Diameters of eggs and oil globules from largemouth bass broodstock fed an unaltered commercial diet (Diet 1) or those top sprayed with fish oil/microalgae oil without (Diet 2) or with nucleotides (Diet 3). Pa rame t e r D i e t 1 D i e t 2 D i e t 3 F p Egg diameter (mm) 1.27 ± 0.23b 1.30 ± 0.21b 1.41 ± 0.06a 10.765 <0.01 Oil globule (mm) 0.52 ± 0.10b 0.54 ± 0.11ab 0.57 ± 0.05a 5.969 0.003 Values (mean ± SD) with the same letter in the row are not significantly different. Di e t 1 – commer c i a l f eed Di e t 2 – commer c i a l f eed + f i sh o i l (0.5 per cent ) + DHA o i l (0.5 per cent ) Di e t 3 – commer c i a l f eed + f i sh o i l (0.5 per cent ) + DHA o i l (0.5 per cent ) + nuc l eot ides (400 mg/kg) TABLE 3. Production from largemouth bass broodstock fed an unaltered commercial diet (Diet 1) or those top sprayed with fish oil/microalgae oil without (Diet 2) or with nucleotides (Diet 3). Pa rame t e r D i e t 1 D i e t 2 D i e t 3 To t a l Number of spawns 45 39 50 134 Total number of fry 57,129 150,324 129,422 336,875 Total number of fingerlings 54,184 94,718 84,620 233,522 Survival from fry to fingerlings (%) 94.8 63.0 65.4 TABLE 1. Proximate composition (percent dry weight) and fatty acid composition (mg/g on as is basis) of the diets fed to largemouth bass Micropterus salmoides broodstock. Di e t 1 Di e t 2 Di e t 3 Moisture 8.30 8.38 8.44 Crude protein 50.29 50.55 51.82 Crude lipid 15.04 15.89 15.03 Crude ash 9.29 9.34 9.41 Crude fiber 1.50 1.63 1.33 Fatty acids C14:0 3.81 4.52 4.63 Myristoleic (9c-14:1) 0.04 0.06 0.04 C15:0 0.36 0.37 0.36 Palmitic (16:0) 19.33 19.16 19.28 Palmitoleic (9c-16:1) 5.73 5.84 5.83 Margaric (17:0) 0.08 0.07 0.07 Stearic (18:0) 5.21 4.98 5.01 Oleic (9c-18:1) 21.68 20.84 20.78 Vaccenic (11c-18:1) 2.30 2.27 2.27 Linoleic (18:2n6) 19.50 18.84 18.41 Linolenic (18:3n3) 1.87 1.85 1.85 g-Linolenic (C18:3n6) 0.21 0.21 0.21 Arachidic (20:0) 0.40 0.38 0.38 Gonodic (20:1n9) 0.74 0.72 0.72 C20:2 0.10 0.11 0.11 Arachidonic (20:4n6) 0.98 0.95 0.95 EPA (20:5n3) 4.58 4.73 4.65 Erucic (22:1n9) 0.44 0.42 0.41 C22:2n6 0.45 0.47 0.48 Clupanodonic (22:5n3) 0.92 0.92 0.92 DHA (22:6n3) 3.65 4.39 4.52 Di e t 1 – c omme r c i a l f e ed Di e t 2 – c omme r c i a l f e ed + f i s h o i l (0 . 5 p e r c en t) + DHA o i l (0 . 5 p e r c en t) Di e t 3 – c omme r c i a l f e ed + f i s h o i l (0 . 5 p e r c en t) + DHA o i l (0 . 5 p e r c en t) + nu c l e o t i de s (400 mg /kg )
RkJQdWJsaXNoZXIy MjExNDY=